In order to get it dilution was only used to dilutions 10-1, 10-2, 10-3 in the analyses of the samples. It was used methodology proposal for Hisses et al. (2001) that it was the technique of the multiple pipes for the most likely number (NMP/g). 2,3 2.3.1 MICROBIOLOGICAL DETERMINATION Determination of the Most likely Number of Coliformes Total (NMP/g) For the determination of Coliformes total used it technique of multiple pipes for the most likely number (NMP/g). It made it dilution 10-1, homogeneizando 25g and cheese with 225mL of peptonada water 1% and the seriadas dilutions (10-2, 10-3), sowed 1mL of each dilution, in third copy.
The used way was Caldo Lauril Sulfato Triptose (LST) and incubou it 35C during 24 and 48 hours. From the reading of the combination it enters the positive pipes (with growth and production of gas in the pipes of Durham) and negatives was determined the NMP/g for total coliformes. It became confirmation with Green Broth Shining Bile 2% in the same conditions of time and temperature praised by the American Public Health Association (APHA, 1992). 2.3.2 Determination of the Most likely Number of Coliformes Termotolerantes technique of the multiple pipes had been quantified using it, with Escherichia Broth coli (EC). Of total the positive pipes for Coliformes, the repicagens of the culture had become, for pipes with Broth EC. After the sowing incubou it 44,5oC for 24? 48 hours, in bath? Maria, with series of three pipes for each dilution praised for the American Public Health Association (APHA, 1992).
From the reading of the combination it enters the positive pipes (with growth and production of gas in the pipes of Durham) and negatives was determined the NMP/g for termotolerantes coliformes.
The Gabriel method answers us extensively the question you would like I can lose weight fast and safely? Gabriel explains that our bodies have a switch that determines our weight. Ignore the internal logic of our bodies has the potential to condemn us to be obese’s lifetime. Some tricks in order to turn off the fat switch are: 1. be sure to include the right foods in your diet so that your body can count with the necessary nutrients. The food that we eat today has been processed so that it no longer counts with the vast majority of the original nutrients. This makes your body creates there is scarcity of food and the FAT mechanism activated. One of the main reasons why diets don’t work is that your body responds in the same way when he refrains from eating.
Jon Gabriel discusses this topic in more detail in his book the Gabriel method. 2. Drink water more often. Your body often desperately needed water and instead provide you with what you need, people confuse this with cravings and end up eating instead. This has its logic in that many foods in their original form contain water, so when the body is dehydrated, sends signals of hunger, in order to obtain water through food. Try to drink water before meals and on any occasion that it be provided with a craving. The frequency with which the sensation of hunger decreases by drinking water 3 is amazing. Be creative when it comes to exercising: choose a series of exercises that can be performed virtually anywhere.
There is one small technique that helps you achieve the best possible results: trot at normal pace for 10 minutes, then run with all their forces for 30 seconds as if someone or something was chasing it, then repeat the same process. This gives your primitive brain the sense of danger and convinces him that to survive you needed to be thin. The Gabriel method is extremely effective and has scientific backing, in addition, it has the advantage that the changes offered are usually permanent since it promotes a change of habits instead of diets. Discover the Gabriel method.
Hot compresses before the suck ones facilitate the exit of milk. To suckle frequently, to manually extract milk or with suction bomb. To use analgesics, if necessary. Table 3 – Measures for Treatment of the Mammary Ingurgitamento. Source: Periodical of Pediatrics 1995 (GIUGLIANE) 2.3.2 TRAUMAS MAMILARES.
A time installed, the traumas mamilares are very painful and many times hinder the mothers to suckle. The predisponent factors of traumas mamilares would be the lubricant use during the prenatal one, excess of hygiene of the mamilos preceding or succeeding breast-feeding, the mammary ingurgitamento and defects of suction in the just-been born one (FREITAS, 2001). The attempt of preparation of the mamilos during the prenatal one with massages, lubricant and measures of hygiene provokes the removal of hidrolipdica membrane that protects the region, promoting the scalling off in the crnea layer of the skin, passing the pathogenic flora to dominate in substitution to the normal flora. ' ' suction not eficiente' ' also mamilares can be cause of traumas; the child suck during 24 hours per day, with interval very short (between 45 minutes and one hour). The complaints maternas are: hipersensveis mamilos, doloridos and ardidos, associates to the presence of petquias, vesicles and isquemia in the region mamilo to aureole. The behavior to space the schedule enters the suck ones for three or four hours are efficient in the recovery of the mamilos and correction of the habit of vicious suction in the child (SAINTS, 2000). Figures 5 and 6? Mamilares traumas. Source: access in 19/08/2009.
Useful measures in the treatment of the traumas mamilares include: Correction of the techniques of breast-feeding, whenever error of technique is detected. Positions alternated in the suck ones. Milk application in the mamilos after the suck ones. Although not to have given scientific that bases this behavior, to many they recommend it specialists. Maternal milk has many antiinfectious properties, what it diminishes the risk of secondary infection.
When one starts to read about the law of attraction is easy to understand what is said, however is no longer so easy to understand what you want to convey (why most applied everything you read and then say abra cadabra and the rabbit does not appear). I.e. it is as if one at first when she begins to read is reading a text in Ukrainian and reading is going to grow in quantity, gradually some of those same words are going translating alone Spanish. You understand what I mean? I am referring to that when already past few months absorb readings of dozens of books and hundreds of pages about the law of attraction, is as if the same concept that was previously read in another book, now seen with more experience sees it another way and understanding that you get now is different to its first readingeven when the words remain the same. Why many authors say that you should read the books several times, because with each new reading wisdom that contains every one of them is releasing in major quantity. And there comes a time when one understands the true meaning which brings with it the law of attraction. It is said that it goes beyond the definition itself attract our lives directly proportionally to that kind of thoughts we have positive or negative events. It is there that is then seen that second meaning that I say, that meaning that is deeper, hidden to the readings that are started, not because it is more or less intelligent, the subject does not pass by there, but because still in that starting point we did not make that change of thought (from the competitive to the creative) that will allow us to open our minds to new knowledge. Why is that that turning point arrives when one understands that the law of attraction is nothing more than an instruction manual of how to interpret the Scriptures.
Are you unhappy with the appearance of your face? Do you have scars, acne problems, or your face begins to show the first signs of aging? If so, you may be curious regarding all options that exist. Despite the fact that you have a number of options in different treatments, many men and women in your place opt for cosmetic surgery. Therefore, should you get a surgical facelift? Of course, there are a number of factors that you must take into account when considering it. There are, however, signs that you’ll want to see. If these signs, five of which are described below, apply to you, a facelift may be your best interest. 1.
You’re getting old it is no secret that our skin changes as we age. Unfortunately, these changes are not always pleasant and attractive. In the course of your life, you can have developed small scars on the face. These can be maintained through surgery cosmetics. Although there are a number of reasons why you might submit to a cosmetic surgery, treatment for wrinkles is the most common. It is important to remember that just because you’re doing you older doesn’t mean you look so – 2 you embarrassing you ashamed for the way in which you look? Although each is beautiful in its own way, that does not help to change the way in how you feel. Those who undergo cosmetic surgery often have as sole purpose the improve your appearance. If you’re depressed with the signs of aging, if your face is loose after a big loss of weight, or just want a change, a cosmetic facelift surgery can be an option to make you consider it seriously. 3 Already you’ve reviewed other options as pleasant as it is to improve your appearance by subjecting you to a surgical facelift, it is important to know that there are other options.